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ProductsBack/GSTA4 Mouse Monoclonal Antibody [Clone ID: OTI3F5]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSTA4 (Cat# RC202130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTA4(Cat# TA501903). Positive lysates [LY400591] (100ug) and [LC400591] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSTA4 (Cat# RC202130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTA4(Cat# TA501903). Positive lysates [LY400591] (100ug) and [LC400591] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GSTA4 (Cat# RC202130, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSTA4(Cat# TA501903). Positive lysates [LY400591] (100ug) and [LC400591] (20ug) can be purchased separately from OriGene.

GSTA4 Mouse Monoclonal Antibody [Clone ID: OTI3F5]

CF501903
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGSTA4
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GSTA4 Mouse Monoclonal Antibody [Clone ID: OTI3F5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F5
  • Gene ID2941
  • Target name
    GSTA4
  • Target description
    glutathione S-transferase alpha 4
  • Target synonyms
    GSTA4-4, GTA4, glutathione S-transferase A4, GST class-alpha member 4, S-(hydroxyalkyl)glutathione lyase A4, glutathione S-alkyltransferase A4, glutathione S-aralkyltransferase A4, glutathione S-aryltransferase A4, glutathione S-transferase A4-4, glutathione transferase A4-4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO15217
  • Protein Name
    Glutathione S-transferase A4
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GSTA4 mouse monoclonal antibody, clone OTI3F5 (formerly 3F5)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of GSTA4 using anti-GSTA4 antibody (A03699-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SH-SY5Y lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTA4 antigen affinity purified polyclonal antibody (Catalog # A03699-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTA4 at approximately 26KD. The expected band size for GSTA4 is at 26KD.
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