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ProductsBack/GSTP1 antibody [3F2C2]
FACS analysis of K562 cells using GTX83077 GSTP1 antibody [3F2C2]. Green : GSTP1 Purple : negative control
FACS analysis of K562 cells using GTX83077 GSTP1 antibody [3F2C2]. Green : GSTP1 Purple : negative control
FACS analysis of K562 cells using GTX83077 GSTP1 antibody [3F2C2]. Green : GSTP1 Purple : negative control

GSTP1 antibody [3F2C2]

GTX83077
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGSTP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GSTP1 antibody [3F2C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F2C2
  • Conjugate
    Unconjugated
  • Gene ID2950
  • Target name
    GSTP1
  • Target description
    glutathione S-transferase pi 1
  • Target synonyms
    deafness, X-linked 7; DFN7; epididymis secretory protein Li 22; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; GST class-pi; GST3; GSTP; GSTP1-1; HEL-S-22; PI
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09211
  • Protein Name
    Glutathione S-transferase P
  • Scientific Description
    Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of GST3/GST pi using anti-GST3/GST pi antibody (PB9184). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GST3/GST pi antigen affinity purified polyclonal antibody (Catalog # PB9184) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GST3/GST pi at approximately 23 kDa. The expected band size for GST3/GST pi is at 23 kDa.
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