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ProductsBack/HAO1 Mouse Monoclonal Antibody [Clone ID: OTI5A1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HAO1 (Cat# RC216834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1(Cat# TA501959). Positive lysates [LY413692] (100ug) and [LC413692] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HAO1 (Cat# RC216834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1(Cat# TA501959). Positive lysates [LY413692] (100ug) and [LC413692] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HAO1 (Cat# RC216834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1(Cat# TA501959). Positive lysates [LY413692] (100ug) and [LC413692] (20ug) can be purchased separately from OriGene.

HAO1 Mouse Monoclonal Antibody [Clone ID: OTI5A1]

CF501959
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHAO1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HAO1 Mouse Monoclonal Antibody [Clone ID: OTI5A1]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5A1
  • Gene ID54363
  • Target name
    HAO1
  • Target description
    hydroxyacid oxidase 1
  • Target synonyms
    GO, GOX, GOX1, HAOX1, 2-Hydroxyacid oxidase 1, (S)-2-hydroxy-acid oxidase, glycolate oxidase 1, glyoxylate oxidase, hydroxyacid oxidase (glycolate oxidase) 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UJM8
  • Protein Name
    2-Hydroxyacid oxidase 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone OTI5A1 (formerly 5A1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HAO1 using anti-HAO1 antibody (A09159-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO1 antigen affinity purified polyclonal antibody (Catalog # A09159-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO1 at approximately 41 kDa. The expected band size for HAO1 is at 41 kDa.
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