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ProductsBack/HAS1 / HAS Antibody (aa171-220, HRP)

HAS1 / HAS Antibody (aa171-220, HRP)

LS-C470877
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHAS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HAS1 / HAS Antibody (aa171-220, HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.65 mg/ml
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID3036
  • Target name
    HAS1
  • Target description
    hyaluronan synthase 1
  • Target synonyms
    HA synthase 1; HAS; hyaluronan synthase 1; hyaluronate synthase 1; hyaluronic acid synthase 1
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Immunofluorescence staining of A549 cells with CSB-PA010139LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Figure 1. Western blot analysis of HAS1 using anti-HAS1 antibody (A04784-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SHG-44 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat smooth muscle tissue lysates, Lane 5: rat ovary tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse smooth muscle tissue lysates, Lane 8: mouse ovary tissue lysates, Lane 9: mouse small intestine tissue lysates, Lane 10: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAS1 antigen affinity purified polyclonal antibody (Catalog # A04784-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAS1 at approximately 70KD. The expected band size for HAS1 is at 65KD.
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