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HAVCR2 Antibody Pair

CSB-EAP010145
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Goat, Human, Porcine
TargetHAVCR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HAVCR2 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.5ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID84868
  • Target name
    HAVCR2
  • Target description
    hepatitis A virus cellular receptor 2
  • Target synonyms
    CD366; HAVcr-2; hepatitis A virus cellular receptor 2; kidney injury molecule-3; KIM-3; SPTCL; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM3; Tim-3; TIMD3; TIMD-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TDQ0
  • Protein Name
    Hepatitis A virus cellular receptor 2
  • Reactivity
    Bovine, Canine, Chicken, Equine, Guinea Pig, Goat, Human, Porcine
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of TIM 3 using anti-TIM 3 antibody (A00657-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 3 antigen affinity purified polyclonal antibody (Catalog # A00657-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 3 at approximately 45-70KD. The expected band size for TIM 3 is at 33KD.
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