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Western Blot analysis of Hela cell using HCN1 Polyclonal Antibody at dilution of 1:950
Western Blot analysis of Hela cell using HCN1 Polyclonal Antibody at dilution of 1:950
Western Blot analysis of Hela cell using HCN1 Polyclonal Antibody at dilution of 1:950

HCN1 Polyclonal Antibody

E-AB-15708
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HCN1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    The membrane protein encoded by this gene is a hyperpolarization-activated cation channel that contributes to the native pacemaker currents in heart and neurons. The encoded protein can homodimerize or heterodimerize with other pore-forming subunits to form a potassium channel. This channel may act as a receptor for sour tastes.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HCN1 using anti-HCN1 antibody (PB9210). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCN1 antigen affinity purified polyclonal antibody (Catalog # PB9210) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCN1 at approximately 120 kDa. The expected band size for HCN1 is at 99 kDa.
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