ChIP analysis of U-2OS cells using GTX20012 HDAC1 antibody. Left : ChIP results using the anti-HDAC1 antibody (bar 2) or beads only (bar 1) and PCR primers specific for p21 Right : ChIP results using the anti-HDAC1 antibody (bar 4) or beads only (bar 3) and PCR primers for GAPDH (used as negative control). ChIP reaction : 2.4microg antibody / 1 million cells
ChIP analysis of U-2OS cells using GTX20012 HDAC1 antibody. Left : ChIP results using the anti-HDAC1 antibody (bar 2) or beads only (bar 1) and PCR primers specific for p21 Right : ChIP results using the anti-HDAC1 antibody (bar 4) or beads only (bar 3) and PCR primers for GAPDH (used as negative control). ChIP reaction : 2.4microg antibody / 1 million cells
HDAC1 antibody
GTX20012
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDAC1
Overview
- SupplierGeneTex
- Product NameHDAC1 antibody
- Delivery Days Customer9
- Application Supplier NoteICC/IF: 1:200. ELISA: 1:100 - 1:300. ChIP assay: 2.4 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID3065
- Target nameHDAC1
- Target descriptionhistone deacetylase 1
- Target synonymsGON-10; HD1; histone deacetylase 1; KDAC1; reduced potassium dependency, yeast homolog-like 1; RPD3; RPD3L1
- HostRabbit
- IsotypeIgG
- Protein IDQ13547
- Protein NameHistone deacetylase 1
- Scientific DescriptionHistone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203