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ProductsBack/HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDAC1 (Cat# RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1 (Cat# TA502193). Positive lysates [LY417624] (100ug) and [LC417624] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDAC1 (Cat# RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1 (Cat# TA502193). Positive lysates [LY417624] (100ug) and [LC417624] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HDAC1 (Cat# RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1 (Cat# TA502193). Positive lysates [LY417624] (100ug) and [LC417624] (20ug) can be purchased separately from OriGene.

HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9]

CF502193
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetHDAC1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5F9]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F9
  • Gene ID3065
  • Target name
    HDAC1
  • Target description
    histone deacetylase 1
  • Target synonyms
    GON-10, HD1, KDAC1, RPD3, RPD3L1, histone deacetylase 1, protein deacetylase HDAC1, protein deacylase HDAC1, protein decrotonylase HDAC1, reduced potassium dependency, yeast homolog-like 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ13547
  • Protein Name
    Histone deacetylase 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HDAC1 mouse monoclonal antibody, clone OTI5F9 (formerly 5F9)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC1 antigen affinity purified polyclonal antibody (Catalog # A00256-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC1 at approximately 65 kDa. The expected band size for HDAC1 is at 65 kDa.
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