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Raji lysates probed with HDAC1/HD1 Polyclonal Antibody, unconjugated (bs-1414R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Raji lysates probed with HDAC1/HD1 Polyclonal Antibody, unconjugated (bs-1414R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
Raji lysates probed with HDAC1/HD1 Polyclonal Antibody, unconjugated (bs-1414R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

HDAC1 Polyclonal Antibody

BS-1414R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHDAC1
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Overview

  • Supplier
    Bioss
  • Product Name
    HDAC1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3065
  • Target name
    HDAC1
  • Target description
    histone deacetylase 1
  • Target synonyms
    GON-10, HD1, KDAC1, RPD3, RPD3L1, histone deacetylase 1, protein deacetylase HDAC1, protein deacylase HDAC1, protein decrotonylase HDAC1, reduced potassium dependency, yeast homolog-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13547
  • Protein Name
    Histone deacetylase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li H, Hou L, Zhang Y, et al. PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway. Cells. 2019,8(9). doi: 10.3390/cells8090959
    Read this paper

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Figure 1. Western blot analysis of HDAC1 using anti-HDAC1 antibody (A00256-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC1 antigen affinity purified polyclonal antibody (Catalog # A00256-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC1 at approximately 65 kDa. The expected band size for HDAC1 is at 65 kDa.
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