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Anti-Histone Deacetylase 2 (Phospho-S394) Antibody

ORB515513
Biorbyt
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-Histone Deacetylase 2 (Phospho-S394) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB: 1:500-1000 WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3066
  • Target name
    HDAC2
  • Target description
    histone deacetylase 2
  • Target synonyms
    HD2, KDAC2, RPD3, YAF1, histone deacetylase 2, YY1-associated factor 1, protein deacylase HDAC2, transcriptional regulator homolog RPD3
  • Host
    Rabbit
  • Protein IDP70288
  • Protein Name
    Histone deacetylase 2
  • Scientific Description
    Rabbit polyclonal antibody to HDAC2 (phospho-S394)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of HDAC2 using anti-HDAC2 antibody (A00325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat pancreas tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified polyclonal antibody (Catalog # A00325-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 60 kDa.
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