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Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue All lanes: HDAC8 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 30, 18, 32, 29, 16, 17 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue All lanes: HDAC8 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 30, 18, 32, 29, 16, 17 kDa Observed band size: 42 kDa
Western Blot Positive WB detected in: Rat heart tissue, Rat liver tissue All lanes: HDAC8 antibody at 2.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 30, 18, 32, 29, 16, 17 kDa Observed band size: 42 kDa

HDAC8 Antibody

CSB-PA010244EA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetHDAC8
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC8 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55869
  • Target name
    HDAC8
  • Target description
    histone deacetylase 8
  • Target synonyms
    CDA07, CDLS5, HD8, HDACL1, KDAC8, MRXS6, RPD3, WTS, histone deacetylase 8, histone deacetylase-like 1, protein deacetylase HDAC8, protein decrotonylase HDAC8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BY41
  • Protein Name
    Histone deacetylase 8
  • Scientific Description
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Also involved in the deacetylation of cohesin complex protein SMC3 regulating release of cohesin complexes from chromatin. May play a role in smooth muscle cell contractility.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC8 using anti-HDAC8 antibody (A01843-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 antigen affinity purified polyclonal antibody (Catalog # A01843-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC8 at approximately 50 kDa. The expected band size for HDAC8 is at 42 kDa.
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