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Immunohistochemistry of paraffin-embedded human thyroid cancer using CSB-PA245265 at dilution of 1:20
Immunohistochemistry of paraffin-embedded human thyroid cancer using CSB-PA245265 at dilution of 1:20
Immunohistochemistry of paraffin-embedded human thyroid cancer using CSB-PA245265 at dilution of 1:20

HES1 Antibody

CSB-PA245265
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHES1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HES1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3280
  • Target name
    HES1
  • Target description
    hes family bHLH transcription factor 1
  • Target synonyms
    bHLHb39; class B basic helix-loop-helix protein 39; hairy homolog; hairy-like protein; HES-1; HHL; HRY; transcription factor HES-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14469
  • Protein Name
    Transcription factor HES-1
  • Scientific Description
    This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hes1 using anti-Hes1 antibody (M01459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hes1 antigen affinity purified monoclonal antibody (Catalog # M01459) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hes1 at approximately 30 kDa. The expected band size for Hes1 is at 30 kDa.
Product group Antibodies
References
Boster Bio
Anti-Hes1 Rabbit Monoclonal AntibodyM01459
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetHES1
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Product group Antibodies
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Anti-HES1 Antibody144-62113
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TargetHES1
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References
GeneTex
HES1 antibody [N1], N-termGTX108356
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetHES1
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Product group Antibodies
Biomatik
Hairy And Enhancer Of Split 1 (HES1) Polyclonal AntibodyCAU21580
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetHES1
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HES1 Polyclonal Antibody, Unconjugated (bs-2972R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss Antibodies
HES1 Polyclonal AntibodyBS-2972R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetHES1
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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Product group Antibodies
Atlas Antibodies
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ReactivityHuman
TargetHES1
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HES1 / HES-1 AntibodyLS-C747087
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TargetHES1
  • SizePrice
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HES1 Monoclonal AntibodyCAB0925
TargetHES1
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Product group Antibodies
MedChem Express
Hes1 Antibody (YA387, Rabbit)HY-P80155
TargetHES1
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