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ProductsBack/HES1 antibody [N1], N-term

HES1 antibody [N1], N-term

GTX108356
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
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Product group Antibodies
TargetHES1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HES1 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.18 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3280
  • Target name
    HES1
  • Target description
    hes family bHLH transcription factor 1
  • Target synonyms
    bHLHb39; class B basic helix-loop-helix protein 39; hairy homolog; hairy-like protein; HES-1; HHL; HRY; transcription factor HES-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14469
  • Protein Name
    Transcription factor HES-1
  • Scientific Description
    This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ginsenoside Rg3 Suppresses Epithelial-Mesenchymal Transition via Downregulating Notch-Hes1 Signaling in Colon Cancer Cells. Li X et al., 2021, Am J Chin Med
    Read more
  • MicroRNA-223-induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways. Liu Z et al., 2021 Feb, Mol Med Rep
    Read more
  • Transcription Repressor Hes1 Contributes to Neuropathic Pain Development by Modifying CDK9/RNAPII-Dependent Spinal mGluR5 Transcription. Hsieh MC et al., 2019 Aug 26, Int J Mol Sci
    Read more
  • Salvianolic Acid B Enhances Hepatic Differentiation of Human Embryonic Stem Cells Through Upregulation of WNT Pathway and Inhibition of Notch Pathway. Chen J et al., 2018 Feb 15, Stem Cells Dev
    Read more
  • Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein beta (C/EBPbeta) expression. Obora K et al., 2017 Feb 27, Sci Rep
    Read more
  • Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45alpha expression to increase cell survival. Chiou HY et al., 2014 Jun 4, J Biomed Sci
    Read more
  • Notch-1 signaling promotes the malignant features of human breast cancer through NF-kappaB activation. Li L et al., 2014, PLoS One
    Read more

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Figure 1. Western blot analysis of Hes1 using anti-Hes1 antibody (M01459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hes1 antigen affinity purified monoclonal antibody (Catalog # M01459) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hes1 at approximately 30 kDa. The expected band size for Hes1 is at 30 kDa.
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