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ProductsBack/HES1 antibody [N1], N-term

HES1 antibody [N1], N-term

GTX108356
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetHES1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HES1 antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.18 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3280
  • Target name
    HES1
  • Target description
    hes family bHLH transcription factor 1
  • Target synonyms
    HES-1, HHL, HRY, bHLHb39, transcription factor HES-1, class B basic helix-loop-helix protein 39, hairy homolog, hairy-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14469
  • Protein Name
    Transcription factor HES-1
  • Scientific Description
    This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Li X, Liu W, Geng C, et al. Ginsenoside Rg3 Suppresses Epithelial-Mesenchymal Transition via Downregulating Notch-Hes1 Signaling in Colon Cancer Cells. Am J Chin Med. 2021,49(1):217-235. doi: 10.1142/S0192415X21500129
    Read this paper
  • Liu Z, Ma T, Duan J, et al. MicroRNA‑223‑induced inhibition of the FBXW7 gene affects the proliferation and apoptosis of colorectal cancer cells via the Notch and Akt/mTOR pathways. Mol Med Rep. 2021,23(2):pii: 154. doi: 10.3892/mmr.2020.11793.
    Read this paper
  • Hsieh MC, Peng HY, Ho YC, et al. Transcription Repressor Hes1 Contributes to Neuropathic Pain Development by Modifying CDK9/RNAPII-Dependent Spinal mGluR5 Transcription. Int J Mol Sci. 2019,20(17). doi: 10.3390/ijms20174177
    Read this paper
  • Chen J, Tschudy-Seney B, Ma X, et al. Salvianolic Acid B Enhances Hepatic Differentiation of Human Embryonic Stem Cells Through Upregulation of WNT Pathway and Inhibition of Notch Pathway. Stem Cells Dev. 2018,27(4):252-261. doi: 10.1089/scd.2017.0168
    Read this paper
  • Obora K, Onodera Y, Takehara T, et al. Inflammation-induced miRNA-155 inhibits self-renewal of neural stem cells via suppression of CCAAT/enhancer binding protein β (C/EBPβ) expression. Sci Rep. 2017,7:43604. doi: 10.1038/srep43604
    Read this paper
  • Chiou HY, Liu SY, Lin CH, et al. Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45α expression to increase cell survival. J Biomed Sci. 2014,21(1):53. doi: 10.1186/1423-0127-21-53
    Read this paper
  • Li L, Zhao F, Lu J, et al. Notch-1 signaling promotes the malignant features of human breast cancer through NF-κB activation. PLoS One. 2014,9(4):e95912. doi: 10.1371/journal.pone.0095912
    Read this paper

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Figure 1. Western blot analysis of Hes1 using anti-Hes1 antibody (M01459). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U-87MG whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hes1 antigen affinity purified monoclonal antibody (Catalog # M01459) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hes1 at approximately 30 kDa. The expected band size for Hes1 is at 30 kDa.
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