Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![HES1 antibody [N1], N-term detects HES1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 protein stained by HES1 antibody [N1], N-term (GTX108356) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108356/GTX108356_39946_20150410_IFA_w_23060120_240.webp "HES1 antibody [N1], N-term detects HES1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HES1 protein stained by HES1 antibody [N1], N-term (GTX108356) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.")
![HES1 antibody [N1], N-term detects HES1 protein by western blot analysis. A. 30 μg PC-12 whole cell extract 12% SDS-PAGE HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108356/GTX108356_39946_WB_R_w_23060120_237.webp "HES1 antibody [N1], N-term detects HES1 protein by western blot analysis. A. 30 μg PC-12 whole cell extract 12% SDS-PAGE HES1 antibody [N1], N-term (GTX108356) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HES1 antibody [N1], N-term (GTX108356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
HES1 antibody [N1], N-term
GTX108356
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHES1
Overview
- SupplierGeneTex
- Product NameHES1 antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:2000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.18 mg/ml
- ConjugateUnconjugated
- Gene ID3280
- Target nameHES1
- Target descriptionhes family bHLH transcription factor 1
- Target synonymsHES-1, HHL, HRY, bHLHb39, transcription factor HES-1, class B basic helix-loop-helix protein 39, hairy homolog, hairy-like protein
- HostRabbit
- IsotypeIgG
- Protein IDQ14469
- Protein NameTranscription factor HES-1
- Scientific DescriptionThis protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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