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ProductsBack/Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3E6]
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human Hex plasmid (RC204815, hHex), mouse Hex plasmid (MR203607, mHex) using anti-Hex antibody TA500219 (1:100). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human Hex plasmid (RC204815, hHex), mouse Hex plasmid (MR203607, mHex) using anti-Hex antibody TA500219 (1:100). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)
Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human Hex plasmid (RC204815, hHex), mouse Hex plasmid (MR203607, mHex) using anti-Hex antibody TA500219 (1:100). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3E6]

CF500219
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHHEX
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3E6]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3E6
  • Gene ID3087
  • Target name
    HHEX
  • Target description
    hematopoietically expressed homeobox
  • Target synonyms
    HEX, HMPH, HOX11L-PEN, PRH, PRHX, hematopoietically-expressed homeobox protein HHEX, homeobox protein HEX, homeobox protein PRH, homeobox, hematopoietically expressed, proline-rich homeodomain protein, proline-rich homeodomain-containing transcription factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ03014
  • Protein Name
    Hematopoietically-expressed homeobox protein HHEX
  • Scientific Description
    Carrier-free (BSA/glycerol-free) Hex mouse monoclonal antibody, clone OTI3E6 (formerly 3E6)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hex using anti-Hex antibody (PB9631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hex antigen affinity purified polyclonal antibody (Catalog # PB9631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hex at approximately 40 kDa. The expected band size for Hex is at 40 kDa.
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