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ProductsBack/Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3F4 1B1]
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HHEX-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HHEX monoclonal antibody TA500099(1:10000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HHEX-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HHEX monoclonal antibody TA500099(1:10000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.
Equivalent amounts of cell lysates (30 ug per lane) of wild-type HeLa cells(WT) and HHEX-Knockdown HeLa cells(KD) were separated by SDS-PAGE and immunoblotted with anti-HHEX monoclonal antibody TA500099(1:10000).Then the blotted membrane was stripped and reprobed with anti-HSP90AA1 antibody as a loading control.

Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3F4 1B1]

CF500099
OriGene
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHHEX
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Hex (HHEX) Mouse Monoclonal Antibody [Clone ID: OTI3F4 1B1]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3F4 1B1
  • Gene ID3087
  • Target name
    HHEX
  • Target description
    hematopoietically expressed homeobox
  • Target synonyms
    HEX, HMPH, HOX11L-PEN, PRH, PRHX, hematopoietically-expressed homeobox protein HHEX, homeobox protein HEX, homeobox protein PRH, homeobox, hematopoietically expressed, proline-rich homeodomain protein, proline-rich homeodomain-containing transcription factor
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ03014
  • Protein Name
    Hematopoietically-expressed homeobox protein HHEX
  • Scientific Description
    Carrier-free (BSA/glycerol-free) Hex mouse monoclonal antibody, clone OTI3F4 1B1 (formerly 3F4 1B1)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Hex using anti-Hex antibody (PB9631). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hex antigen affinity purified polyclonal antibody (Catalog # PB9631) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hex at approximately 40 kDa. The expected band size for Hex is at 40 kDa.
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