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ProductsBack/Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5]
Western blot analysis of extracts (30ug per lane) from 4 different cell lines lysates by using anti-Hexokinase II antibody(TA500856,1:1000@1mg/ml).
Western blot analysis of extracts (30ug per lane) from 4 different cell lines lysates by using anti-Hexokinase II antibody(TA500856,1:1000@1mg/ml).
Western blot analysis of extracts (30ug per lane) from 4 different cell lines lysates by using anti-Hexokinase II antibody(TA500856,1:1000@1mg/ml).

Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5]

CF500856
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetHK2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Hexokinase II (HK2) Mouse Monoclonal Antibody [Clone ID: OTI4C5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4C5
  • Gene ID3099
  • Target name
    HK2
  • Target description
    hexokinase 2
  • Target synonyms
    HKII, HXK2, hexokinase-2, hexokinase type II, hexokinase-2, muscle, hexokinase-B, muscle form hexokinase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP52789
  • Protein Name
    Hexokinase-2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HK2 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HK2 using anti-HK2 antibody (A01389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HK2 antigen affinity purified polyclonal antibody (Catalog # A01389) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HK2 at approximately 102 kDa. The expected band size for HK2 is at 102 kDa.
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