Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody (GTX118007) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 protein stained by HEY1 antibody (GTX118007) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX118007/GTX118007_40870_20150410_IFA_w_23060519_680.webp "HEY1 antibody detects HEY1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HEY1 protein stained by HEY1 antibody (GTX118007) diluted at 1:2000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Hoechst 33342 staining.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody (GTX118007) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HEY1 antibody
GTX118007
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetHEY1
Overview
- SupplierGeneTex
- Product NameHEY1 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.04 mg/ml
- ConjugateUnconjugated
- Gene ID23462
- Target nameHEY1
- Target descriptionhes related family bHLH transcription factor with YRPW motif 1
- Target synonymsBHLHb31, CHF2, HERP2, HESR1, HRT-1, NERP2, OAF1, hHRT1, hairy/enhancer-of-split related with YRPW motif protein 1, HES-related repressor protein 1, HES-related repressor protein 2, basic helix-loop-helix protein OAF1, cardiovascular helix-loop-helix factor 2, class B basic helix-loop-helix protein 31, hairy and enhancer of split-related protein 1, hairy-related transcription factor 1, hairy/enhancer-of-split related with YRPW motif 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y5J3
- Protein NameHairy/enhancer-of-split related with YRPW motif protein 1
- Scientific DescriptionThis gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Wei ST, Huang YC, Hsieh ML, et al. Atypical chemokine receptor ACKR3/CXCR7 controls postnatal vasculogenesis and arterial specification by mesenchymal stem cells via Notch signaling. Cell Death Dis. 2020,11(5):307. doi: 10.1038/s41419-020-2512-2Read this paper
Datasheet
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