Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638573/GTX638573_T-45033_20230512_WB_R_lung_23051702_480.webp "Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HEY1 stained by HEY1 antibody [HL2367] (GTX638573) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX638573/GTX638573_T-45033_20230512_IHC-P_M_23060622_956.webp "HEY1 antibody [HL2367] detects HEY1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. HEY1 stained by HEY1 antibody [HL2367] (GTX638573) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638573/GTX638573_45117_20230728_WB_23073119_935.webp "Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638573/GTX638573_45117_20230908_WB_D_23091319_729.webp "Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638573/GTX638573_45117_20230908_WB_Z_23091319_797.webp "Whole zebrafish extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with HEY1 antibody [HL2367] (GTX638573) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HEY1 antibody [HL2367]
GTX638573
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat, Zebra Fish
TargetHEY1
Overview
- SupplierGeneTex
- Product NameHEY1 antibody [HL2367]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2367
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23462
- Target nameHEY1
- Target descriptionhes related family bHLH transcription factor with YRPW motif 1
- Target synonymsBHLHb31, CHF2, HERP2, HESR1, HRT-1, NERP2, OAF1, hHRT1, hairy/enhancer-of-split related with YRPW motif protein 1, HES-related repressor protein 1, HES-related repressor protein 2, basic helix-loop-helix protein OAF1, cardiovascular helix-loop-helix factor 2, class B basic helix-loop-helix protein 31, hairy and enhancer of split-related protein 1, hairy-related transcription factor 1, hairy/enhancer-of-split related with YRPW motif 1
- HostRabbit
- IsotypeIgG
- Protein IDQ9Y5J3
- Protein NameHairy/enhancer-of-split related with YRPW motif protein 1
- Scientific DescriptionThis gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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