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ProductsBack/HIF2 alpha antibody [ep190b]
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate

HIF2 alpha antibody [ep190b]

GTX30123
GeneTex
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetEPAS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HIF2 alpha antibody [ep190b] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 - 2 microg/ml. IHC-P: 1:150 - 1:300. FACS: 1:400. ELISA: 1:100 - 1:2000. IHC: 1:150 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ep190b
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2034
  • Target name
    EPAS1
  • Target description
    endothelial PAS domain protein 1
  • Target synonyms
    basic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99814
  • Protein Name
    Endothelial PAS domain-containing protein 1
  • Scientific Description
    This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction. Nagalakshmi VK et al., 2018 Dec 12, Clin Sci (Lond)
    Read more
  • Thiamine deficiency activates hypoxia inducible factor-1alpha to facilitate pro-apoptotic responses in mouse primary astrocytes. Zera K et al., 2017, PLoS One
    Read more
  • VHL and PTEN loss coordinate to promote mouse liver vascular lesions. Chen S et al., 2010 Mar, Angiogenesis
    Read more
  • Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma. Wright TM et al., 2010 Apr 23, J Biol Chem
    Read more
  • ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress. Myers K et al., 2009 Jan, PLoS Genet
    Read more
  • VHL type 2B mutations retain VBC complex form and function. Hacker KE et al., 2008, PLoS One
    Read more
  • Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Catrina SB et al., 2006 Aug 1, Clin Cancer Res
    Read more

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Figure 1. Western blot analysis of HIF-2-Alpha/EPAS1 using anti-HIF-2-Alpha/EPAS1 antibody (A01248-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-2-Alpha/EPAS1 antigen affinity purified polyclonal antibody (Catalog # A01248-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HIF-2-Alpha/EPAS1 at approximately 96KD. The expected band size for HIF-2-Alpha/EPAS1 is at 96KD.
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