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WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate

HIF2 alpha antibody [ep190b]

GTX30123

ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Product group Antibodies

ReactivityHamster, Human, Mouse, Rat

TargetEPAS1

Overview

Supplier
GeneTex
Product Name
HIF2 alpha antibody [ep190b]
Delivery Days Customer
9
Application Supplier Note
WB: 1 - 2 microg/ml. IHC-P: 1:150 - 1:300. FACS: 1:400. ELISA: 1:100 - 1:2000. IHC: 1:150 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
Applications
Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Certification
Research Use Only
Clonality
Monoclonal
Clone ID
ep190b
Concentration
1 mg/ml
Conjugate
Unconjugated
Gene ID2034
Target name
EPAS1
Target description
endothelial PAS domain protein 1
Target synonyms
ECYT4, HIF2A, HLF, MOP2, PASD2, bHLHe73, endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, HIF-1alpha-like factor, HIF-2-alpha, HIF2-alpha, PAS domain-containing protein 2, basic-helix-loop-helix-PAS protein MOP2, class E basic helix-loop-helix protein 73, hypoxia-inducible factor 2 alpha, member of PAS protein 2
Host
Mouse
Isotype
IgG1
Protein IDQ99814
Protein Name
Endothelial PAS domain-containing protein 1
Scientific Description
This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
Reactivity
Hamster, Human, Mouse, Rat
Storage Instruction
-20°C or -80°C,2°C to 8°C
UNSPSC
12352203

References

Nagalakshmi VK, Li M, Shah S, et al. Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction. Clin Sci (Lond). 2018,132(23):2519-2545. doi: 10.1042/CS20180623
Read this paper
Zera K, Zastre J. Thiamine deficiency activates hypoxia inducible factor-1α to facilitate pro-apoptotic responses in mouse primary astrocytes. PLoS One. 2017,12(10):e0186707. doi: 10.1371/journal.pone.0186707
Read this paper
Chen S, Sanford CA, Sun J, et al. VHL and PTEN loss coordinate to promote mouse liver vascular lesions. Angiogenesis. 2010,13(1):59-69. doi: 10.1007/s10456-010-9164-2
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Wright TM, Rathmell WK. Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma. J Biol Chem. 2010,285(17):12916-24. doi: 10.1074/jbc.M109.073924
Read this paper
Myers K, Gagou ME, Zuazua-Villar P, et al. ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress. PLoS Genet. 2009,5(1):e1000324. doi: 10.1371/journal.pgen.1000324
Read this paper
Hacker KE, Lee CM, Rathmell WK. VHL type 2B mutations retain VBC complex form and function. PLoS One. 2008,3(11):e3801. doi: 10.1371/journal.pone.0003801
Read this paper
Catrina SB, Botusan IR, Rantanen A, et al. Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Clin Cancer Res. 2006,12(15):4506-14.
Read this paper

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HIF2 alpha antibody [ep190b]

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