![WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX30123/GTX30123_1335_WB_w_23060722_677.webp)
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
HIF2 alpha antibody [ep190b]
GTX30123
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetEPAS1
Overview
- SupplierGeneTex
- Product NameHIF2 alpha antibody [ep190b] - Orthogonal Validated
- Delivery Days Customer9
- Antibody SpecificityThis is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
- Application Supplier NoteWB: 1 - 2 microg/ml. IHC-P: 1:150 - 1:300. FACS: 1:400. ELISA: 1:100 - 1:2000. IHC: 1:150 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDep190b
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID2034
- Target nameEPAS1
- Target descriptionendothelial PAS domain protein 1
- Target synonymsbasic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2
- HostMouse
- IsotypeIgG1
- Protein IDQ99814
- Protein NameEndothelial PAS domain-containing protein 1
- Scientific DescriptionThis gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
- ReactivityHamster, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction. Nagalakshmi VK et al., 2018 Dec 12, Clin Sci (Lond)Read more
- Thiamine deficiency activates hypoxia inducible factor-1alpha to facilitate pro-apoptotic responses in mouse primary astrocytes. Zera K et al., 2017, PLoS OneRead more
- VHL and PTEN loss coordinate to promote mouse liver vascular lesions. Chen S et al., 2010 Mar, AngiogenesisRead more
- Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma. Wright TM et al., 2010 Apr 23, J Biol ChemRead more
- ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress. Myers K et al., 2009 Jan, PLoS GenetRead more
- VHL type 2B mutations retain VBC complex form and function. Hacker KE et al., 2008, PLoS OneRead more
- Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Catrina SB et al., 2006 Aug 1, Clin Cancer ResRead more