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WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate
WB analysis of hypoxia-treated A549 cell lysate using GTX30123 HIF2 alpha antibody [ep190b]. Lane 1: normoxic A549 lysate control Lane 2: hypoxic A549 lysate

HIF2 alpha antibody [ep190b]

GTX30123
GeneTex
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetEPAS1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HIF2 alpha antibody [ep190b] - Orthogonal Validated
  • Delivery Days Customer
    9
  • Antibody Specificity
    This is specific for HIF-2 alpha and does not cross-react with HIF-1 alpha.
  • Application Supplier Note
    WB: 1 - 2 microg/ml. IHC-P: 1:150 - 1:300. FACS: 1:400. ELISA: 1:100 - 1:2000. IHC: 1:150 - 1:300. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ep190b
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2034
  • Target name
    EPAS1
  • Target description
    endothelial PAS domain protein 1
  • Target synonyms
    basic-helix-loop-helix-PAS protein MOP2; bHLHe73; class E basic helix-loop-helix protein 73; ECYT4; endothelial PAS domain-containing protein 1; EPAS-1; HIF-1-alpha-like factor; HIF-1alpha-like factor; HIF2A; HIF-2-alpha; HIF2-alpha; HLF; hypoxia-inducible factor 2 alpha; member of PAS protein 2; MOP2; PAS domain-containing protein 2; PASD2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99814
  • Protein Name
    Endothelial PAS domain-containing protein 1
  • Scientific Description
    This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq]
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Changes in cell fate determine the regenerative and functional capacity of the developing kidney before and after release of obstruction. Nagalakshmi VK et al., 2018 Dec 12, Clin Sci (Lond)
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  • Thiamine deficiency activates hypoxia inducible factor-1alpha to facilitate pro-apoptotic responses in mouse primary astrocytes. Zera K et al., 2017, PLoS One
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  • VHL and PTEN loss coordinate to promote mouse liver vascular lesions. Chen S et al., 2010 Mar, Angiogenesis
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  • Identification of Ror2 as a hypoxia-inducible factor target in von Hippel-Lindau-associated renal cell carcinoma. Wright TM et al., 2010 Apr 23, J Biol Chem
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  • ATR and Chk1 suppress a caspase-3-dependent apoptotic response following DNA replication stress. Myers K et al., 2009 Jan, PLoS Genet
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  • VHL type 2B mutations retain VBC complex form and function. Hacker KE et al., 2008, PLoS One
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  • Hypoxia-inducible factor-1alpha and hypoxia-inducible factor-2alpha are expressed in kaposi sarcoma and modulated by insulin-like growth factor-I. Catrina SB et al., 2006 Aug 1, Clin Cancer Res
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