ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60813 Histone H2A.Z (acetyl Lys4/Lys7/Lys11) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. Quantitative PCR was performed with primers specific for the promoter of the active genes CCT5 and EIF4A2, used as positive controls, and for the coding region of the inactive MYT1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).