ChIP analysis of sheared chromatin from 1.6x10? U2OS cells using GTX60344 Histone H3R17me2 (Asymmetric Di-methyl Arg17) antibody - ChIP grade. A titration of the antibody consisting of 2, 5, 10 and 15 microl per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). Figure 1A: QPCR performed with primers for the GAPDH promoter and for exon 2 of the myoglobin gene. Figure 1B: QPCR performed with primers for the promoter of the active ALDOA gene and for the coding region of the inactive MYOD gene..