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ProductsBack/Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade
ChIP analysis of sheared chromatin from 10? K562 cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? K562 cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? K562 cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 microg per ChIP experiment was analysed. IgG (1 microg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade

GTX60337
GeneTex
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman, Mouse
TargetH4C1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1,000. ICC/IF: 1:500. Dot: 1:20,000. ELISA: 1:1,000. ChIP assay: 0.5-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Dot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.76 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8359
  • Target name
    H4C1
  • Target description
    H4 clustered histone 1
  • Target synonyms
    H4 histone family, member A; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FA; HIST1H4A; histone 1, H4a; histone cluster 1 H4 family member a; histone cluster 1, H4a; histone H4
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chromatin profiling in human neurons reveals aberrant roles for histone acetylation and BET family proteins in schizophrenia. Farrelly LA et al., 2022 Apr 22, Nat Commun
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