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Western Blot analysis of A431, K562, 231, hela and hepG2 cell using HK2 Polyclonal Antibody at dilution of 1:1200
Western Blot analysis of A431, K562, 231, hela and hepG2 cell using HK2 Polyclonal Antibody at dilution of 1:1200
Western Blot analysis of A431, K562, 231, hela and hepG2 cell using HK2 Polyclonal Antibody at dilution of 1:1200

HK2 Polyclonal Antibody

E-AB-14706
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HK2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HK2 using anti-HK2 antibody (A01389). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HK2 antigen affinity purified polyclonal antibody (Catalog # A01389) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HK2 at approximately 102 kDa. The expected band size for HK2 is at 102 kDa.
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