More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human HNF6 plasmid (RC218219, hHNF6), mouse HNF6 plasmid (MR207440, mHNF6) using anti-HNF6 antibody TA500196 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human HNF6 plasmid (RC218219, hHNF6), mouse HNF6 plasmid (MR207440, mHNF6) using anti-HNF6 antibody TA500196 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (5ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human HNF6 plasmid (RC218219, hHNF6), mouse HNF6 plasmid (MR207440, mHNF6) using anti-HNF6 antibody TA500196 (1:20000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]

CF500196
OriGene
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetONECUT1
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    HNF6 (ONECUT1) Mouse Monoclonal Antibody [Clone ID: OTI4F12]
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4F12
  • Gene ID3175
  • Target name
    ONECUT1
  • Target description
    one cut homeobox 1
  • Target synonyms
    HNF-6, HNF6, HNF6A, hepatocyte nuclear factor 6, hepatocyte nuclear factor 6, alpha, one cut domain family member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UBC0
  • Protein Name
    Hepatocyte nuclear factor 6
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HNF6 mouse monoclonal antibody, clone OTI4F12 (formerly 4F12)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-ONECUT1 Antibody144-12774
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetONECUT1
  • SizePrice
Figure 1. Western blot analysis of ONECUT1 using anti-ONECUT1 antibody (A07349). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ONECUT1 antigen affinity purified polyclonal antibody (Catalog # A07349) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ONECUT1 at approximately 50 kDa. The expected band size for ONECUT1 is at 51,51-55 kDa.
Product group Antibodies
Boster Bio
Anti-ONECUT1 Antibody Picoband(r)A07349-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetONECUT1
  • SizePrice
Anti-HNF6 Antibody
Product group Antibodies
Antibodies.com
Anti-HNF6 AntibodyA90316
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ONECUT1 / HNF6 AntibodyLS-C830150
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetONECUT1
  • SizePrice
Immunohistochemistry analysis in human liver and small intestine tissues using Anti-ONECUT1 antibody. Corresponding ONECUT1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-ONECUT1 AntibodyHPA003457
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetONECUT1
  • SizePrice
Western Blot Positive WB detected in: Jurkat whole cell lysate, Rat brain tissue All lanes: ONECUT1 antibody at 7.6ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
Product group Antibodies
Cusabio
ONECUT1 AntibodyCSB-PA880058LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetONECUT1
  • SizePrice
Product group Antibodies
Biomatik
ONECUT1 Polyclonal AntibodyCAC15332
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityRat
TargetONECUT1
  • SizePrice
Product group Antibodies
Bioss
HNF6 Polyclonal AntibodyBS-18062R
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Equine, Human, Mouse, Rat, Sheep
TargetONECUT1
  • SizePrice
HepG2 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HNF6 antibody (GTX134403) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
HNF6 antibodyGTX134403
ApplicationsWestern Blot
ReactivityHuman
TargetONECUT1
  • SizePrice