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HNRNPH1 Antibody

CSB-PA010609GA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHNRNPH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRNPH1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3187
  • Target name
    HNRNPH1
  • Target description
    heterogeneous nuclear ribonucleoprotein H1
  • Target synonyms
    HNRPH, HNRPH1, NEDCDS, hnRNPH, heterogeneous nuclear ribonucleoprotein H, epididymis secretory sperm binding protein, heterogeneous nuclear ribonucleoprotein H1 (H)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31943
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein H
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HnRNP H/HNRNPH1 using anti-HnRNP H/HNRNPH1 antibody (A07691). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human LNCAP whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human RT4 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNP H/HNRNPH1 antigen affinity purified polyclonal antibody (Catalog # A07691) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNP H/HNRNPH1 at approximately 49 kDa. The expected band size for HnRNP H/HNRNPH1 is at 49 kDa.
Product group Antibodies
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ReactivityHuman, Mouse, Rat
TargetHNRNPH1
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Western blot All lanes: HNRNPH1 antibody at 8microg/ml Lane 1: HepG2 whole cell lysate Lane 2: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 50 kDa Observed band size: 50 kDa
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ReactivityHuman
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TargetHNRNPH1
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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with hnRNP H antibody [N1C1] (GTX102061) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
hnRNP H antibody [N1C1]GTX102061
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
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