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ProductsBack/HNRPH1 Antibody Pair

HNRPH1 Antibody Pair

CSB-EAP04264
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine
TargetHNRNPH1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HNRPH1 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.3ug/ml and the detection antibody at a concentration of 0.5ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID3187
  • Target name
    HNRNPH1
  • Target description
    heterogeneous nuclear ribonucleoprotein H1
  • Target synonyms
    epididymis secretory sperm binding protein; heterogeneous nuclear ribonucleoprotein H; heterogeneous nuclear ribonucleoprotein H1 (H); hnRNPH; HNRPH; HNRPH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31943
  • Protein Name
    Heterogeneous nuclear ribonucleoprotein H
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Mouse, Porcine
  • UNSPSC
    41116163

FAQ

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Figure 1. Western blot analysis of HnRNP H/HNRNPH1 using anti-HnRNP H/HNRNPH1 antibody (A07691). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human LNCAP whole cell lysates, Lane 6: human U2OS whole cell lysates, Lane 7: human RT4 whole cell lysates, Lane 8: rat C6 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HnRNP H/HNRNPH1 antigen affinity purified polyclonal antibody (Catalog # A07691) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HnRNP H/HNRNPH1 at approximately 49 kDa. The expected band size for HnRNP H/HNRNPH1 is at 49 kDa.
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