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Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using HOXB1 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using HOXB1 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using HOXB1 Polyclonal Antibody at dilution 1:40

HOXB1 Polyclonal Antibody

E-AB-10371
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HOXB1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXB genes located in a cluster on chromosome 17.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HOXB1 using anti-HOXB1 antibody (A04724-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate,Lane 2: mouse testis tissue lysate,Lane 3: human MCF-7 whole Cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB1 antigen affinity purified polyclonal antibody (Catalog # A04724-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB1 at approximately 32KD. The expected band size for HOXB1 is at 32KD.
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