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Western blot analysis of extracts from K562 cells, using HOXB9 antibody.
Western blot analysis of extracts from K562 cells, using HOXB9 antibody.
Western blot analysis of extracts from K562 cells, using HOXB9 antibody.

HOXB9 Antibody

CSB-PA062208
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetHOXB9
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Overview

  • Supplier
    Cusabio
  • Product Name
    HOXB9 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3219
  • Target name
    HOXB9
  • Target description
    homeobox B9
  • Target synonyms
    HOX-2.5, HOX2, HOX2E, homeobox protein Hox-B9, homeo box 2E, homeo box B9, homeobox protein Hox-2.5, homeobox protein Hox-2E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17482
  • Protein Name
    Homeobox protein Hox-B9
  • Scientific Description
    Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Kosaki K., Teratology 65:50-62(2002). Giampaolo A., Differentiation 40:191-197(1989). Boncinelli E., Genome 31:745-756(1989).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HOXB9 using anti-HOXB9 antibody (A05689-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXB9 antigen affinity purified polyclonal antibody (Catalog # A05689-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HOXB9 at approximately 32KD. The expected band size for HOXB9 is at 32KD.
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