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HPD antibody

70R-2553
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    HPD antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western blot All lanes: HPD antibody at 4.5microg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 41 kDa Observed band size: 45 kDa
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HPD antibody [N1C2] (GTX103994) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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Figure 1. Western blot analysis of HPD using anti-HPD antibody (A02838-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPD antigen affinity purified polyclonal antibody (Catalog # A02838-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPD at approximately 45 kDa. The expected band size for HPD is at 45 kDa.
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