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HPD Antibody

LS-C334758
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHPD
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HPD Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa/44kDa, while the observed MW by Western blot was 45kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 40kDa/44kDa, while the observed MW by Western blot was 45kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3242
  • Target name
    HPD
  • Target description
    4-hydroxyphenylpyruvate dioxygenase
  • Target synonyms
    4HPPD; 4-HPPD; 4-hydroxyphenylpyruvate dioxygenase; 4-hydroxyphenylpyruvic acid oxidase; GLOD3; glyoxalase domain containing 3; HPPDASE; PPD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: HPD antibody at 4.5microg/ml Lane 1: Mouse kidney tissue Lane 2: Mouse liver tissue Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45, 41 kDa Observed band size: 45 kDa
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Figure 1. Western blot analysis of HPD using anti-HPD antibody (A02838-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HPD antigen affinity purified polyclonal antibody (Catalog # A02838-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HPD at approximately 45 kDa. The expected band size for HPD is at 45 kDa.
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