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WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54013 Hsp40 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54013 Hsp40 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54013 Hsp40 antibody. Dilution : 1:1000 Loading : 25microg per lane

Hsp40 antibody

GTX54013
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetDNAJB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Hsp40 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3337
  • Target name
    DNAJB1
  • Target description
    DnaJ heat shock protein family (Hsp40) member B1
  • Target synonyms
    DnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ homolog subfamily B member 1; dnaJ protein homolog 1; Hdj1; heat shock 40 kDa protein 1; Hsp40; HSPF1; human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25685
  • Protein Name
    DnaJ homolog subfamily B member 1
  • Scientific Description
    This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the J-domain and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: 293T cell lysates; Lane 2: MCF-7 lysates; Lane 3: BxPC-3 cell lysates; Lane 4: BT-474 cell lysates; Lane 5: SW620 cell lysates; Lane 6: K562 cell lysates; Lane 7: Mouse testis lysates; Lane 8: Mouse spleen lysates; Lane 9: Mouse thymus lysates probed with DNAJB1 Polyclonal Antibody, Unconjugated (bs-55055R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Hsp40/DNAJB1 using anti-Hsp40/DNAJB1 antibody (A03100-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp40/DNAJB1 antigen affinity purified polyclonal antibody (Catalog # A03100-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp40/DNAJB1 at approximately 38 kDa. The expected band size for Hsp40/DNAJB1 is at 38 kDa.
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Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
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