Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Cell lysates of Jurkat (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hsp40 (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Hsp40 antibody [k1C7]
GTX50059
Overview
- SupplierGeneTex
- Product NameHsp40 antibody [k1C7]
- Delivery Days Customer9
- Application Supplier NoteWe recommend the following starting dilutions:Western Blot: Use at 1:1,000 ~ 2,000.Optimal working concentrations should be determined experimentally by the end user.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDk1C7
- Concentration1 mg/ml
- ConjugateUnconjugated
- FormulationLiquid
- Gene ID3337
- Target nameDNAJB1
- Target descriptionDnaJ heat shock protein family (Hsp40) member B1
- Target synonymsDnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ homolog subfamily B member 1; dnaJ protein homolog 1; Hdj1; heat shock 40 kDa protein 1; Hsp40; HSPF1; human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1
- HostMouse
- IsotypeIgG2b
- Protein IDP25685
- Protein NameDnaJ homolog subfamily B member 1
- Scientific DescriptionThis gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the J-domain and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203