HSP40 Antibody: Biotin

Biorbyt

HSP40 antibody

16

0.5 microg/ml of SPC-100 was sufficient for detection of HSP40 in 20 microg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry

WB (1:2000), ICC/IF (1:100) ELISA, ICC, IF, IHC, WB

Research Use Only

Polyclonal

Biotin

DNAJB1

DnaJ heat shock protein family (Hsp40) member B1

DnaJ (Hsp40) homolog, subfamily B, member 1; dnaJ homolog subfamily B member 1; dnaJ protein homolog 1; Hdj1; heat shock 40 kDa protein 1; Hsp40; HSPF1; human DnaJ protein 1; radial spoke 16 homolog B; RSPH16B; Sis1

Rabbit

DnaJ homolog subfamily B member 1

Rabbit polyclonal to Hsp40 (Biotin). DnaJ/Hsp40 proteins have been preserved throughout evolution and are important for protein translation, folding, unfolding, translocation, and degradation, primarily by stimulating the ATPase activity of chaperone proteins, Hsp70s. Because the ATP hydrolysis is essential for the activity of Hsp70s, DnaJ/Hsp40 proteins actually determine the activity of Hsp70s by stabilizing their interaction with substrate proteins. DnaJ/Hsp40 proteins all contain the J domain through which they bind to Hsp70s. Hsp40, also known as HDJ1, is a basic mammalian 40kDa heat shock protein which is not only homologous to the bacterial heat shock protein (DnaJ), but also yeast DnaJ-related proteins such as SCJ1, Sec63/Npl1, YDJ1 and SIS1 (2-5). Hsp 40 is inducible by stress including heat after which is moves from the cytoplasm to the nucleus and nucleoli; an intracellular pattern similar to Hsc70/Hsp70, the mammalian homologues of the bacterial heat shock protein, DnaK..

Human

See Manual

12352203