![IHC-P analysis of lung cancer tissue (left) and kidney cancer tissue (right) using GTX60424 HSP60 antibody [3G8]. IHC-P analysis of lung cancer tissue (left) and kidney cancer tissue (right) using GTX60424 HSP60 antibody [3G8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_IHC-P_1_w_23061123_246.webp)
IHC-P analysis of lung cancer tissue (left) and kidney cancer tissue (right) using GTX60424 HSP60 antibody [3G8].
HSP60 antibody [3G8]
GTX60424
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetHSPD1
Overview
- SupplierGeneTex
- Product NameHSP60 antibody [3G8]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3G8
- ConjugateUnconjugated
- Gene ID3329
- Target nameHSPD1
- Target descriptionheat shock protein family D (Hsp60) member 1
- Target synonymsCPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13, 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, P60 lymphocyte protein, chaperonin 60, epididymis secretory sperm binding protein, heat shock 60kDa protein 1 (chaperonin), heat shock protein 65, heat shock protein family D member 1, mitochondrial matrix protein P1, short heat shock protein 60 Hsp60s1
- HostMouse
- IsotypeIgG1
- Protein IDP10809
- Protein Name60 kDa heat shock protein, mitochondrial
- Scientific DescriptionThis gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. [provided by RefSeq, Jun 2010]
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ELISA analysis of antigen using GTX60424 HSP60 antibody [3G8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng ELISA analysis of antigen using GTX60424 HSP60 antibody [3G8].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ELISA_w_23061123_845.webp)
![WB analysis of T47D (1), HeLa (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60424 HSP60 antibody [3G8]. WB analysis of T47D (1), HeLa (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH3T3 (7), PC-12 (8) and Cos7 (9) cell lysate using GTX60424 HSP60 antibody [3G8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_WB_w_23061123_843.webp)
![FACS analysis of HeLa cells using GTX60424 HSP60 antibody [3G8]. Green : HSP60 Purple : negative control FACS analysis of HeLa cells using GTX60424 HSP60 antibody [3G8]. Green : HSP60 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_FACS_w_23061123_352.webp)
![ICC/IF analysis of 3T3-L1 cells using GTX60424 HSP60 antibody [3G8]. Green : HSP60 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of 3T3-L1 cells using GTX60424 HSP60 antibody [3G8]. Green : HSP60 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_ICCIF_w_23061123_848.webp)
![IHC-P analysis of breast cancer tissue (left) and colon cancer tissue (right) using GTX60424 HSP60 antibody [3G8]. IHC-P analysis of breast cancer tissue (left) and colon cancer tissue (right) using GTX60424 HSP60 antibody [3G8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60424/GTX60424_20170912_IHC-P_w_23061123_754.webp)
![WB analysis of HeLa whole cell lysate using GTX02649 HSP60 antibody [HSPD1/2206R].](https://www.genetex.com/upload/website/prouct_img/normal/GTX02649/GTX02649_20210319_WB_w_23053122_319.webp)




![ICC/IF analysis of HeLa cells using GTX25478 HSP60 antibody [4B9/89]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25478/GTX25478_661_ICC-IF_w_23060722_354.webp)
![ICC/IF analysis of A431 cells using GTX25479 HSP60 antibody [2E1/53]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25479/GTX25479_668_ICC-IF_w_23060722_504.webp)
