Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![HSP70 1L antibody [N1N3] detects HSP70 1L protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:400. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111045/GTX111045_40058_20160706_IFA_w_23060500_137.webp "HSP70 1L antibody [N1N3] detects HSP70 1L protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSP70 1L protein stained by HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:400. Blue: Hoechst 33342 staining.")
![Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111045/GTX111045_40058_20160303_WB_M_testis_w_23060500_436.webp "Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111045/GTX111045_40058_20160303_WB_R_testis_w_23060500_431.webp "Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [N1N3] (GTX111045) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
HSP70 1L antibody [N1N3]
GTX111045
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA1L
Overview
- SupplierGeneTex
- Product NameHSP70 1L antibody [N1N3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3305
- Target nameHSPA1L
- Target descriptionheat shock protein family A (Hsp70) member 1 like
- Target synonymsHSP70-1L, HSP70-HOM, HSP70T, hum70t, heat shock 70 kDa protein 1-like, epididymis secretory sperm binding protein, heat shock 10kDa protein 1-like, heat shock 70 kDa protein 1-Hom, heat shock 70 kDa protein 1L, heat shock 70kD protein-like 1, heat shock 70kDa protein 1-like, heat shock protein family A member 1L
- HostRabbit
- IsotypeIgG
- Protein IDP34931
- Protein NameHeat shock 70 kDa protein 1-like
- Scientific DescriptionThis gene encodes a 70kDa heat shock protein. In conjunction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which also encode isoforms of the 70kDa heat shock protein. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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