IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
![IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1290_IHC-P_w_23060722_915.webp "IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50")
![IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1291_IHC-P_w_23060722_939.webp "IHC-P analysis of human tonsil tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1444_IP_w_23060722_297.webp "IP analysis of HeLa cell lysates using GTX25444 Hsp70 antibody [4G4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1777_WB_w_23060722_611.webp "WB analysis of 50 ug of the indicated whole cell lysates using GTX25444 Hsp70 antibody [4G4]. Dilution : 1:1000")
![ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_656_ICC-IF_w_23060722_270.webp "ICC/IF analysis of NIH-3T3 cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_657_ICC-IF_w_23060722_490.webp "ICC/IF analysis of HeLa cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_658_ICC-IF_w_23060722_332.webp "ICC/IF analysis of NCI-H1299 Cells using GTX25444 Hsp70 antibody [4G4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20
Hsp70 antibody [4G4]
GTX25444
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
Product group Antibodies
ReactivityHuman, Mouse, Primate
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [4G4]
- Delivery Days Customer9
- Antibody SpecificityThis antibody specifically detects HSP70 and does not cross-react with other members of the HSP70 family. Epitope mapping with a panel of HSP70 deletion mutants suggests that the epitope recognized is located between amino acids 504-617 of human HSP70, a
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:20 - 50. FACS: 1:100 - 1:200. IP: 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4G4
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityHuman, Mouse, Primate
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203