![IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1286_IHC-P_w_23060722_618.webp)
IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Hsp70 antibody [2A4]
GTX25442
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [2A4]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000 - 1:2500. ICC/IF: 1:100-1:200. IHC-P: 1:200. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A4
- Concentration3.5 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, heat shock 70 kDa protein 1A, HSP70-1/HSP70-2, HSP70.1/HSP70.2, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, epididymis secretory sperm binding protein, heat shock 70 kDa protein 1, heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock 70kDa protein 1A, heat shock protein family A member 1A, heat shock-induced protein
- HostMouse
- IsotypeIgM
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure. Moore NS et al., 2021 Mar 12, CellsRead more
![IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1287_IHC-P_w_23060722_416.webp)
![IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1288_IHC-P_w_23060722_497.webp)
![IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000 IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1443_IP_w_23060722_983.webp)
![WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000 WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1775_WB_w_23060722_478.webp)
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000 WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1776_WB_w_23060722_241.webp)
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_648_ICC-IF_w_23060722_208.webp)
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_649_ICC-IF_w_23060722_636.webp)
![ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_650_ICC-IF_w_23060722_689.webp)
![ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_651_ICC-IF_w_23060722_822.webp)
![IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1110_IHC-P_w_23060620_738.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1283_IHC-P_w_23060722_400.webp)
![IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1289_IHC-P_w_23060722_635.webp)

