IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1287_IHC-P_w_23060722_416.webp "IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1288_IHC-P_w_23060722_497.webp "IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1443_IP_w_23060722_983.webp "IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1775_WB_w_23060722_478.webp "WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000")
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1776_WB_w_23060722_241.webp "WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000")
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_648_ICC-IF_w_23060722_208.webp "ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_649_ICC-IF_w_23060722_636.webp "ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_650_ICC-IF_w_23060722_689.webp "ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber")
![ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_651_ICC-IF_w_23060722_822.webp "ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber")
IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Hsp70 antibody [2A4]
GTX25442
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [2A4]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000 - 1:2500. ICC/IF: 1:100-1:200. IHC-P: 1:200. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A4
- Concentration3.5 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgM
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure. Moore NS et al., 2021 Mar 12, CellsRead more
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