IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1287_IHC-P_w_23060722_416.webp "IHC-P analysis of human breast tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1288_IHC-P_w_23060722_497.webp "IHC-P analysis of human tonsil tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1443_IP_w_23060722_983.webp "IP analysis of HeLa cell lysates using GTX25442 Hsp70 antibody [2A4]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1775_WB_w_23060722_478.webp "WB analysis of 30 ug of HEK293T whole cell lysate per well using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000")
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1776_WB_w_23060722_241.webp "WB analysis of 50 ug of the indicated whole cell lysates using GTX25442 Hsp70 antibody [2A4]. Dilution : 1:1000")
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_648_ICC-IF_w_23060722_208.webp "ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_649_ICC-IF_w_23060722_636.webp "ICC/IF analysis of NIH-3T3 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:100 overnight at 4 oC")
![ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_650_ICC-IF_w_23060722_689.webp "ICC/IF analysis of A431 cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber")
![ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_651_ICC-IF_w_23060722_822.webp "ICC/IF analysis of HeLa cells using GTX25442 Hsp70 antibody [2A4]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber")
IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Hsp70 antibody [2A4]
GTX25442
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Drosophila, Fish, Human, Mouse, Primate, Rat, Yeast, Zebra Fish
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [2A4]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70 and, following heat shock, HSP72 from yeast, Drosophila, fish, mouse, avian, amphibian and human samples. Epitope mapping with a panel of HSP
- Application Supplier NoteWB: 1:1000 - 1:2500. ICC/IF: 1:100-1:200. IHC-P: 1:200. IP: 2 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2A4
- Concentration3.5 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgM
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityAmphibian, Avian, Drosophila, Fish, Human, Mouse, Primate, Rat, Yeast, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Critical Effects on Akt Signaling in Adult Zebrafish Brain Following Alterations in Light Exposure. Moore NS et al., 2021 Mar 12, CellsRead more