![IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50 IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1283_IHC-P_w_23060722_400.webp)
IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
Hsp70 antibody [3A3]
GTX25439
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [3A3]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, p75, and following heat shock, HSP72 from yeast, Drosophila, fish, porcine, plant, mouse, avian, arthropod (red claw crayfish and black tige
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:200. IP: 2 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityAmphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica. Avendano-Borromeo B et al., 2019 Feb, Parasitol ResRead more
- Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer. Chang CC et al., 2017 Nov, Obesity (Silver Spring)Read more
- Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. Barreca MM et al., 2017 Jul, J Cell PhysiolRead more
- HSP70 regulates the function of mitotic centrosomes. Fang CT et al., 2016 Oct, Cell Mol Life SciRead more
- Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Ozaydin T et al., 2016 Apr, Microsc Res TechRead more
- A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Yang FM et al., 2015 Oct 15, Biochem JRead more
- Hyperbaric oxygen preconditioning induces tolerance against oxidative injury and oxygen-glucose deprivation by up-regulating heat shock protein 32 in rat spinal neurons. Huang G et al., 2014, PLoS OneRead more