![WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000 WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1773_WB_w_23060722_300.webp)
WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000
Hsp70 antibody [3A3]
GTX25439
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [3A3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:200. IP: 2 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, heat shock 70 kDa protein 1A, HSP70-1/HSP70-2, HSP70.1/HSP70.2, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, epididymis secretory sperm binding protein, heat shock 70 kDa protein 1, heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock 70kDa protein 1A, heat shock protein family A member 1A, heat shock-induced protein
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityAmphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica. Avendano-Borromeo B et al., 2019 Feb, Parasitol ResRead this paper
- Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer. Chang CC et al., 2017 Nov, Obesity (Silver Spring)Read this paper
- Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. Barreca MM et al., 2017 Jul, J Cell PhysiolRead this paper
- HSP70 regulates the function of mitotic centrosomes. Fang CT et al., 2016 Oct, Cell Mol Life SciRead this paper
- Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Ozaydin T et al., 2016 Apr, Microsc Res TechRead this paper
- A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Yang FM et al., 2015 Oct 15, Biochem JRead this paper
- Hyperbaric oxygen preconditioning induces tolerance against oxidative injury and oxygen-glucose deprivation by up-regulating heat shock protein 32 in rat spinal neurons. Huang G et al., 2014, PLoS OneRead this paper
![IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3]. IP reaction : 2μg antibody / 500μg lysate Dilution : 1:1000 IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3]. IP reaction : 2μg antibody / 500μg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1442_IP_w_23060722_270.webp)
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_643_ICC-IF_w_23060722_144.webp)
![ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_642_ICC-IF_w_23060722_978.webp)
![ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_641_ICC-IF_w_23060722_342.webp)
![ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_644_ICC-IF_w_23060722_953.webp)
![IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50 IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1283_IHC-P_w_23060722_400.webp)
![IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200 IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1284_IHC-P_w_23060722_525.webp)
![IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20 IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1285_IHC-P_w_23060722_675.webp)
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000 WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1772_WB_w_23060722_977.webp)




![IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1286_IHC-P_w_23060722_618.webp)
![IHC-P analysis of human breast tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1290_IHC-P_w_23060722_915.webp)