IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
![IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1284_IHC-P_w_23060722_525.webp "IHC-P analysis of human testis tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1285_IHC-P_w_23060722_675.webp "IHC-P analysis of human tonsil tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20")
![IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1442_IP_w_23060722_270.webp "IP analysis of HeLa cell lysates using GTX25439 Hsp70 antibody [3A3]. IP reaction : 2microg antibody / 500microg lysate Dilution : 1:1000")
![WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1772_WB_w_23060722_977.webp "WB analysis of 50 ug of the indicated whole cell lysates using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000")
![WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_1773_WB_w_23060722_300.webp "WB analysis of 20ul of gill tissue lysates from the salt marsh mussel, Guekensia demissa isolated from various coves in Rhode Island (indicated above the lanes) using GTX25439 Hsp70 antibody [3A3]. Dilution : 1:1000")
![ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_641_ICC-IF_w_23060722_342.webp "ICC/IF analysis of MCF-7 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_642_ICC-IF_w_23060722_978.webp "ICC/IF analysis of NIH-3T3 cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_643_ICC-IF_w_23060722_144.webp "ICC/IF analysis of HeLa and NIH3T3 cells using GTX25439 Hsp70 antibody [3A3]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX25439/GTX25439_644_ICC-IF_w_23060722_953.webp "ICC/IF analysis of HeLa cells using GTX25439 Hsp70 antibody [3A3]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
Hsp70 antibody [3A3]
GTX25439
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [3A3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:200. IP: 2 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A3
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica. Avendano-Borromeo B et al., 2019 Feb, Parasitol ResRead more
- Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer. Chang CC et al., 2017 Nov, Obesity (Silver Spring)Read more
- Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. Barreca MM et al., 2017 Jul, J Cell PhysiolRead more
- HSP70 regulates the function of mitotic centrosomes. Fang CT et al., 2016 Oct, Cell Mol Life SciRead more
- Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Ozaydin T et al., 2016 Apr, Microsc Res TechRead more
- A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Yang FM et al., 2015 Oct 15, Biochem JRead more
- Hyperbaric oxygen preconditioning induces tolerance against oxidative injury and oxygen-glucose deprivation by up-regulating heat shock protein 32 in rat spinal neurons. Huang G et al., 2014, PLoS OneRead more
Related products
Product group Antibodies
References
Hsp70 antibody [W27]GTX73850
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPA1A
- SizePrice
Product group Antibodies
References
HSP70 Polyclonal AntibodyBS-0126R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetHSPA1A
- SizePrice
![IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1110_IHC-P_w_23060620_738.webp)
Product group Antibodies
References
Hsp70 antibody [5A5]GTX22787
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetHSPA1A
- SizePrice
Product group Antibodies
References
Hsp70 antibody [W27]GTX23148
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPA1A
- SizePrice
![IHC-P analysis of human prostate carcinoma tissue using GTX25442 Hsp70 antibody [2A4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX25442/GTX25442_1286_IHC-P_w_23060722_618.webp)
Product group Antibodies
References
Hsp70 antibody [2A4]GTX25442
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetHSPA1A
- SizePrice
![IHC-P analysis of human prostate carcinoma tissue using GTX25444 Hsp70 antibody [4G4]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:20](https://www.genetex.com/upload/website/prouct_img/normal/GTX25444/GTX25444_1289_IHC-P_w_23060722_635.webp)
Product group Antibodies
Hsp70 antibody [4G4]GTX25444
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking, Other Application
TargetHSPA1A
- SizePrice
Product group Antibodies
References
Hsp70 antibodyGTX111088
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPA1A
- SizePrice
Product group Antibodies
Hsp70 antibody [HL1580]GTX637059
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetHSPA1A
- SizePrice
Product group Antibodies
HSPA1A Polyclonal AntibodyCAC15126
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetHSPA1A
- SizePrice