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IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50
IHC-P analysis of human breast carcinoma tissue using GTX25439 Hsp70 antibody [3A3]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:50

Hsp70 antibody [3A3]

GTX25439
GeneTex
ApplicationsGel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
TargetHSPA1A
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Overview

  • Supplier
    GeneTex
  • Product Name
    Hsp70 antibody [3A3]
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, p75, and following heat shock, HSP72 from yeast, Drosophila, fish, porcine, plant, mouse, avian, arthropod (red claw crayfish and black tige
  • Application Supplier Note
    WB: 1:1000 - 1:5000. ICC/IF: 1:100. IHC-P: 1:200. IP: 2 microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Gel Shift Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A3
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3303
  • Target name
    HSPA1A
  • Target description
    heat shock protein family A (Hsp70) member 1A
  • Target synonyms
    dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP0DMV8
  • Protein Name
    Heat shock 70 kDa protein 1A
  • Reactivity
    Amphibian, Avian, Drosophila, Fish, Human, Insect, Monkey, Molluscs, Mouse, Plant, Porcine, Primate, Rat, Yeast, Other Species
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica. Avendano-Borromeo B et al., 2019 Feb, Parasitol Res
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  • Caveolin-1 Secreted from Adipose Tissues and Adipocytes Functions as an Adipogenesis Enhancer. Chang CC et al., 2017 Nov, Obesity (Silver Spring)
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  • Extracellular Hsp70 Enhances Mesoangioblast Migration via an Autocrine Signaling Pathway. Barreca MM et al., 2017 Jul, J Cell Physiol
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  • HSP70 regulates the function of mitotic centrosomes. Fang CT et al., 2016 Oct, Cell Mol Life Sci
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  • Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages. Ozaydin T et al., 2016 Apr, Microsc Res Tech
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  • A calreticulin-dependent nuclear export signal is involved in the regulation of liver receptor homologue-1 protein folding. Yang FM et al., 2015 Oct 15, Biochem J
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  • Hyperbaric oxygen preconditioning induces tolerance against oxidative injury and oxygen-glucose deprivation by up-regulating heat shock protein 32 in rat spinal neurons. Huang G et al., 2014, PLoS One
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