Hsp70 antibody [W27]

GeneTex

Hsp70 antibody [W27]

9

Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70.

FACS/IF: Use at an assay dependent dilution. IP(Native and denatured): Use at a concentration of 2microg/mg. WB: Use at a concentration of 0.5-1microg/ml for 2hrs at RT. This antibody detects a band of 73 kDa (constitutive), 72 kDa(inducible), which corresponds to the predicted molecular weight of Hsp 70. IHC(formalin fixed paraffin embedded): Use at a concentration of 2-4microg/ml for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions/concentrations should be determined by the end user.

Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

W27

0.2 mg/ml

Unconjugated

HSPA1A

heat shock protein family A (Hsp70) member 1A

dnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1

Mouse

IgG2a

Heat shock 70 kDa protein 1A

Bovine, Human, Monkey

2°C to 8°C

12352203