IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
![ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_445_ICC-IF_w_23060620_488.webp "ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_442_ICC-IF_w_23060620_421.webp "ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![WB analysis of 50μg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1593_WB_w_23060620_707.webp "WB analysis of 50μg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5]. Dilution : 1:1000")
![IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5]. GTX22787 was used as IP and IB antibody IP reaction : 2μg antibody / 500μg lysate WB antibody Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1432_IP_w_23060620_584.webp "IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5]. GTX22787 was used as IP and IB antibody IP reaction : 2μg antibody / 500μg lysate WB antibody Dilution : 1:1000")
![ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_443_ICC-IF_w_23060620_871.webp "ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_444_ICC-IF_w_23060620_389.webp "ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1111_IHC-P_w_23060620_847.webp "IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1110_IHC-P_w_23060620_738.webp "IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100")
IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200
Hsp70 antibody [5A5]
GTX22787
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Bovine, Drosophila, Fish, Human, Molluscs, Mouse, Rabbit, Rat, Yeast, Other Species
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [5A5]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsHEL-S-103, HSP70, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1, heat shock 70 kDa protein 1A, HSP70-1/HSP70-2, HSP70.1/HSP70.2, Heat shock 70 kDa protein 1B, Heat shock 70 kDa protein 2, dnaK-type molecular chaperone HSP70-1, epididymis secretory protein Li 103, epididymis secretory sperm binding protein, heat shock 70 kDa protein 1, heat shock 70 kDa protein 1/2, heat shock 70 kDa protein 1A/1B, heat shock 70kD protein 1A, heat shock 70kDa protein 1A, heat shock protein family A member 1A, heat shock-induced protein
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityAmphibian, Avian, Bovine, Drosophila, Fish, Human, Molluscs, Mouse, Rabbit, Rat, Yeast, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2. Chamberlain KA et al., 2021 Nov 3, NeuronRead this paper
Datasheet
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