IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
![IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1111_IHC-P_w_23060620_847.webp "IHC-P analysis of human testis tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1112_IHC-P_w_23060620_584.webp "IHC-P analysis of human tonsil tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:200")
![IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5]. GTX22787 was used as IP and IB antibody IP reaction : 2microg antibody / 500microg lysate WB antibody Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1432_IP_w_23060620_584.webp "IP analysis of HeLa cell lysates using GTX22787 Hsp70 antibody [5A5]. GTX22787 was used as IP and IB antibody IP reaction : 2microg antibody / 500microg lysate WB antibody Dilution : 1:1000")
![WB analysis of 50microg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_1593_WB_w_23060620_707.webp "WB analysis of 50microg of the indicated whole cell lysates using GTX22787 Hsp70 antibody [5A5]. Dilution : 1:1000")
![ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_442_ICC-IF_w_23060620_421.webp "ICC/IF analysis of HeLa and NIH3T3 cells using GTX22787 Hsp70 antibody [5A5]. Green : Primary antibody Blue : Nuclei Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes Dilution : 1:50 for at least 1 hour at room temperature")
![ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_443_ICC-IF_w_23060620_871.webp "ICC/IF analysis of C6 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_444_ICC-IF_w_23060620_389.webp "ICC/IF analysis of HeLa cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
![ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC](https://www.genetex.com/upload/website/prouct_img/normal/GTX22787/GTX22787_445_ICC-IF_w_23060620_488.webp "ICC/IF analysis of U251 cells using GTX22787 Hsp70 antibody [5A5]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:200 overnight at 4oC")
IHC-P analysis of human prostate carcinoma tissue using GTX22787 Hsp70 antibody [5A5]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:100
Hsp70 antibody [5A5]
GTX22787
ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityAmphibian, Avian, Bovine, Drosophila, Fish, Human, Molluscs, Mouse, Rabbit, Rat, Yeast, Other Species
TargetHSPA1A
Overview
- SupplierGeneTex
- Product NameHsp70 antibody [5A5]
- Delivery Days Customer9
- Antibody SpecificityThis antibody detects several members of the heat shock protein 70 kDa (HSP70) gene family including HSP70, HSC70, GRP78, and following heat shock, HSP72. It is the first monoclonal antibody reported to react with the ATP binding region of HSP70 and an ep
- Application Supplier NoteWB: 1:250 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsGel Shift Assay, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5A5
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3303
- Target nameHSPA1A
- Target descriptionheat shock protein family A (Hsp70) member 1A
- Target synonymsdnaK-type molecular chaperone HSP70-1; epididymis secretory protein Li 103; epididymis secretory sperm binding protein; heat shock 70 kDa protein 1; heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A; heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1B; Heat shock 70 kDa protein 2; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HEL-S-103; HSP70.1; HSP70.1/HSP70.2; HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70-2; HSP70I; HSP72; HSPA1
- HostMouse
- IsotypeIgG1
- Protein IDP0DMV8
- Protein NameHeat shock 70 kDa protein 1A
- ReactivityAmphibian, Avian, Bovine, Drosophila, Fish, Human, Molluscs, Mouse, Rabbit, Rat, Yeast, Other Species
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2. Chamberlain KA et al., 2021 Nov 3, NeuronRead more