Paraformaldehyde-fixed, paraffin embedded Rat Brian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP90 beta Polyclonal Antibody, Unconjugated (bs-0135R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Brian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with HSP90 beta Polyclonal Antibody, Unconjugated (bs-0135R) at 1:400 overnight at 4°C, DAB staining.
HSP90 beta Polyclonal Antibody
BS-0135R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHSP90AB1
Overview
- SupplierBioss
- Product NameHSP90 beta Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3326
- Target nameHSP90AB1
- Target descriptionheat shock protein 90 alpha family class B member 1
- Target synonymsD6S182, HSP84, HSP90B, HSPC2, HSPCB, heat shock protein HSP 90-beta, HSP90-beta, heat shock 84 kDa, heat shock 90kD protein 1, beta, heat shock protein 90 kDa, heat shock protein 90kDa alpha (cytosolic), class B member 1, heat shock protein 90kDa alpha family class B member 1, heat shock protein family C member 3
- HostRabbit
- IsotypeIgG
- Protein IDP08238
- Protein NameHeat shock protein HSP 90-beta
- Storage Instruction-20°C
- UNSPSC12352203
References
- Bozok Cetintas V, Acikgoz E, Yigitturk G, et al. Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells. Medicine (Baltimore). 2016,95(43):e5150. doi: 10.1097/MD.0000000000005150Read this paper
- Mori Y, Akita K, Tanida S, et al. MUC1 protein induces urokinase-type plasminogen activator (uPA) by forming a complex with NF-κB p65 transcription factor and binding to the uPA promoter, leading to enhanced invasiveness of cancer cells. J Biol Chem. 2014,289(51):35193-204. doi: 10.1074/jbc.M114.586461Read this paper
Datasheet
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