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Hsp90Ab1 Polyclonal Antibody

CAC07147
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHSP90AB1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Hsp90Ab1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3326
  • Target name
    HSP90AB1
  • Target description
    heat shock protein 90 alpha family class B member 1
  • Target synonyms
    D6S182, HSP84, HSP90B, HSPC2, HSPCB, heat shock protein HSP 90-beta, HSP90-beta, heat shock 84 kDa, heat shock 90kD protein 1, beta, heat shock protein 90 kDa, heat shock protein 90kDa alpha (cytosolic), class B member 1, heat shock protein 90kDa alpha family class B member 1, heat shock protein family C member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08238
  • Protein Name
    Heat shock protein HSP 90-beta
  • Scientific Description
    The Hsp90Ab1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of HSP90AB1 using anti-HSP90AB1 antibody (PB9636). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse NIH/3T3 whole cell lysates, Lane 8: mouse ANA-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP90AB1 antigen affinity purified polyclonal antibody (Catalog # PB9636) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP90AB1 at approximately 90 kDa. The expected band size for HSP90AB1 is at 90 kDa.
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