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HSPA1L Antibody

CSB-PA010823GA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA1L
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPA1L Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3305
  • Target name
    HSPA1L
  • Target description
    heat shock protein family A (Hsp70) member 1 like
  • Target synonyms
    HSP70-1L, HSP70-HOM, HSP70T, hum70t, heat shock 70 kDa protein 1-like, epididymis secretory sperm binding protein, heat shock 10kDa protein 1-like, heat shock 70 kDa protein 1-Hom, heat shock 70 kDa protein 1L, heat shock 70kD protein-like 1, heat shock 70kDa protein 1-like, heat shock protein family A member 1L
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP34931
  • Protein Name
    Heat shock 70 kDa protein 1-like
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HSPA1L using anti-HSPA1L antibody (A04286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1L antigen affinity purified polyclonal antibody (Catalog # A04286-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA1L at approximately 70 kDa. The expected band size for HSPA1L is at 70 kDa.
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Anti-HSPA1L Antibody Picoband(r)A04286-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPA1L
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Western Blot Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, L02 whole cell lysate, 293 whole cell lysate, Raji whole cell lysate All lanes: HSPA1L antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71 kDa Observed band size: 71 kDa
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TargetHSPA1L
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Product group Antibodies
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Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HSP70 1L antibody [C1C3] (GTX105164) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
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HSP70 1L antibody [C1C3]GTX105164
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetHSPA1L
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