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ProductsBack/HSPA6 Mouse Monoclonal Antibody [Clone ID: OTI1D12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSPA6 (Cat# RC207795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6(Cat# TA501950). Positive lysates [LY419498] (100ug) and [LC419498] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSPA6 (Cat# RC207795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6(Cat# TA501950). Positive lysates [LY419498] (100ug) and [LC419498] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY HSPA6 (Cat# RC207795, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA6(Cat# TA501950). Positive lysates [LY419498] (100ug) and [LC419498] (20ug) can be purchased separately from OriGene.

HSPA6 Mouse Monoclonal Antibody [Clone ID: OTI1D12]

CF501950
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetHSPA6
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HSPA6 Mouse Monoclonal Antibody [Clone ID: OTI1D12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1D12
  • Gene ID3310
  • Target name
    HSPA6
  • Target description
    heat shock protein family A (Hsp70) member 6
  • Target synonyms
    HSP70B', heat shock 70 kDa protein 6, epididymis secretory sperm binding protein, heat shock 70 kDa protein B', heat shock 70kD protein 6 (HSP70B'), heat shock 70kDa protein 6 (HSP70B'), heat shock protein family A member 6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17066
  • Protein Name
    Heat shock 70 kDa protein 6
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HSPA6 mouse monoclonal antibody, clone OTI1D12 (formerly 1D12)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HSPA6 using anti-HSPA6 antibody (A05402-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat smooth muscle tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse smooth muscle tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA6 antigen affinity purified polyclonal antibody (Catalog # A05402-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPA6 at approximately 71 kDa. The expected band size for HSPA6 is at 71 kDa.
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