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ProductsBack/HSPB2 (F-9) Alexa Fluor(r) 488

HSPB2 (F-9) Alexa Fluor(r) 488

sc-514154AF488
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPB2
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    HSPB2 (F-9) Alexa Fluor(r) 488
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 488
  • Gene ID3316
  • Target name
    HSPB2
  • Target description
    heat shock protein family B (small) member 2
  • Target synonyms
    HSP27, Hs.78846, LOH11CR1K, MKBP, heat shock protein beta-2, DMPK-binding protein, heat shock 27kDa protein 2, heat shock protein family B member 2, heat-shock protein beta-2
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HSPB2 using anti-HSPB2 antibody (PB9598). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug, Lane 2: Rat Skeletal Muscle Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB2 antigen affinity purified polyclonal antibody (Catalog # PB9598) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSPB2 at approximately 20 kDa. The expected band size for HSPB2 is at 20 kDa.
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