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HSPE1 Antibody Pair

CSB-EAP10857
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Guinea Pig, Goat, Human, Mouse, Porcine, Sheep
TargetHSPE1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HSPE1 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.8ug/ml and the detection antibody at a concentration of 0.67ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID3336
  • Target name
    HSPE1
  • Target description
    heat shock protein family E (Hsp10) member 1
  • Target synonyms
    10 kDa chaperonin; 10 kDa heat shock protein, mitochondrial; chaperonin 10; CPN10; early-pregnancy factor; EPF; epididymis secretory sperm binding protein; GROES; heat shock 10kD protein 1 (chaperonin 10); heat shock 10kDa protein 1; HSP10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61604
  • Protein Name
    10 kDa heat shock protein, mitochondrial
  • Reactivity
    Bovine, Guinea Pig, Goat, Human, Mouse, Porcine, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of HSP10 using anti-HSP10 antibody (M06192-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP10 antigen affinity purified monoclonal antibody (Catalog # M06192-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP10 at approximately 11, 13 kDa. The expected band size for HSP10 is at 11 kDa.
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