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ProductsBack/Human G-CSF Antibody : LE/AF (OASB01234)

Human G-CSF Antibody : LE/AF (OASB01234)

OASB01234
Aviva Systems Biology
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCSF3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    Human G-CSF Antibody : LE/AF (OASB01234)
  • Delivery Days Customer
    23
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    BVD13-3A5
  • Concentration
    0.5 mg/ml
  • Gene ID1440
  • Target name
    CSF3
  • Target description
    colony stimulating factor 3
  • Target synonyms
    C17orf33, CSF3OS, GCSF, granulocyte colony-stimulating factor, filgrastim, granulocyte-colony stimulating factor, lenograstim, pluripoietin
  • Host
    Rat
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of G-CSF/CSF3 using anti-G-CSF/CSF3 antibody (PB9563). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G-CSF/CSF3 antigen affinity purified polyclonal antibody (Catalog # PB9563) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G-CSF/CSF3 at approximately 22 kDa. The expected band size for G-CSF/CSF3 is at 22 kDa.
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