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ProductsBack/Purified Anti-Human HLA-DR Antibody [L243]

Purified Anti-Human HLA-DR Antibody [L243]

AGEL0413
Assay Genie
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHLA-DRA
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Purified Anti-Human HLA-DR Antibody [L243]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FCM, WB, IHC, IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3122
  • Target name
    HLA-DRA
  • Target description
    major histocompatibility complex, class II, DR alpha
  • Target synonyms
    HLA-DRA1, HLA class II histocompatibility antigen, DR alpha chain, MHC class II antigen DRA, histocompatibility antigen HLA-DR alpha
  • Host
    Mouse
  • Protein IDP01903
  • Protein Name
    HLA class II histocompatibility antigen, DR alpha chain
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (A01195). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human Ramos whole cell lysates, Lane 4: human HMy2.CIR whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DRA antigen affinity purified polyclonal antibody (Catalog # A01195) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 37 kDa. The expected band size for HLA-DRA is at 29 kDa.
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