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HUMAN IGM Antibody (OAAD00095)

OAAD00095
Aviva Systems Biology
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetCD40LG
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    HUMAN IGM Antibody (OAAD00095)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    B481
  • Concentration
    0.3 mg/ml
  • Format
    Liquid 20mM Tris-borate, 150mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, and 0.05% sodium azide (pH 7.5)
  • Gene ID959
  • Target name
    CD40LG
  • Target description
    CD40 ligand
  • Target synonyms
    CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40 ligand, CD40 antigen ligand, CD40-L, T-B cell-activating molecule, T-cell antigen Gp39, TNF-related activation protein, tumor necrosis factor (ligand) superfamily member 5
  • Host
    Mouse
  • Isotype
    IgG1
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (A01114-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody (Catalog # A01114-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD40L at approximately 19KD. The expected band size for CD40L is at 29KD.
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