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Iba1 antibody detects Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Iba1 antibody detects Iba1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. Iba1 stained by Iba1 antibody (GTX100042) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Iba1 antibody

GTX100042
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAIF1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Iba1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID199
  • Target name
    AIF1
  • Target description
    allograft inflammatory factor 1
  • Target synonyms
    AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor 1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55008
  • Protein Name
    Allograft inflammatory factor 1
  • Scientific Description
    This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Jing Y, Yang D, Bai F, et al. Spinal cord injury-induced gut dysbiosis influences neurological recovery partly through short-chain fatty acids. NPJ Biofilms Microbiomes. 2023,9(1):99. doi: 10.1038/s41522-023-00466-5
    Read this paper
  • Sun MH, Ho TC, Yeh SI, et al. Short peptides derived from pigment epithelium-derived factor attenuate retinal ischemia reperfusion injury through inhibition of apoptosis and inflammatory response in rats. Exp Eye Res. 2024,238:109743. doi: 10.1016/j.exer.2023.109743
    Read this paper
  • Maes ME, Colombo G, Schoot Uiterkamp FE, et al. Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. iScience. 2023,26(10):107780. doi: 10.1016/j.isci.2023.107780
    Read this paper
  • Chan L, Hsu W, Chen KY, et al. Therapeutic Effect of Human Adipocyte-derived Stem Cell-derived Exosomes on a Transgenic Mouse Model of Parkinson's Disease. In Vivo. 2023,37(5):2028-2038. doi: 10.21873/invivo.13300
    Read this paper
  • Yang YL, Lai TW. Chronic Visual Stimulation with LED Light Flickering at 24, 40, or 80 Hz Failed to Reduce Amyloid β Load in the 5XFAD Alzheimer's Disease Mouse Model. eNeuro. 2023,10(8). doi: 10.1523/ENEURO.0189-23.2023
    Read this paper
  • Song WS, Hung TH, Liu SH, et al. Neuroprotection by Abdominal Ultrasound in Lipopolysaccharide-Induced Systemic Inflammation. Int J Mol Sci. 2023,24(11). doi: 10.3390/ijms24119329
    Read this paper
  • Lee YZ, Cheng SH, Chang MY, et al. Neuroprotective Effects of Lactobacillus plantarum PS128 in a Mouse Model of Parkinson's Disease: The Role of Gut Microbiota and MicroRNAs. Int J Mol Sci. 2023,24(7). doi: 10.3390/ijms24076794
    Read this paper
  • Uchi T, Konno S, Kihara H, et al. Siponimod ameliorates experimental autoimmune neuritis. J Neuroinflammation. 2023,20(1):35. doi: 10.1186/s12974-023-02706-z
    Read this paper
  • Tanikawa S, Ebisu Y, Sedlačík T, et al. Engineering of an electrically charged hydrogel implanted into a traumatic brain injury model for stepwise neuronal tissue reconstruction. Sci Rep. 2023,13(1):2233. doi: 10.1038/s41598-023-28870-z
    Read this paper
  • Cabron AS, Borgmeyer U, Richter J, et al. Lack of a protective effect of the Tmem106b "protective SNP" in the Grn knockout mouse model for frontotemporal lobar degeneration. Acta Neuropathol Commun. 2023,11(1):21. doi: 10.1186/s40478-023-01510-3
    Read this paper

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Figure 1. Western blot analysis of Iba1/AIF1 using anti-Iba1/AIF1 antibody (A01394). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Iba1/AIF1 antigen affinity purified polyclonal antibody (Catalog # A01394) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Iba1/AIF1 at approximately 17 kDa. The expected band size for Iba1/AIF1 is at 17 kDa.
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